[
  {
    "Question": "Which molecule is the primary energy currency of the cell?",
    "Answer": "B",
    "Explanation": "ATP (adenosine triphosphate) stores and transfers energy within cells, making it the primary energy currency.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/3/3a/ATP_chemical_structure.svg/1200px-ATP_chemical_structure.svg.png",
    "OptionA": "DNA",
    "OptionB": "ATP",
    "OptionC": "Glucose",
    "OptionD": "NADH",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Energy Molecules",
    "Item": 1,
    "Type": "multiple choice",
    "Path": "Biochemistry/Metabolism"
  },
  {
    "Question": "Which process breaks down glucose to produce energy in the form of ATP?",
    "Answer": "C",
    "Explanation": "Glycolysis is the metabolic pathway that converts glucose into pyruvate, producing ATP and NADH.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/3/3d/Glycolysis_overview.svg/1200px-Glycolysis_overview.svg.png",
    "OptionA": "Photosynthesis",
    "OptionB": "Transcription",
    "OptionC": "Glycolysis",
    "OptionD": "Replication",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Metabolic Pathways",
    "Item": 2,
    "Type": "multiple choice",
    "Path": "Biochemistry/Metabolism"
  },
  {
    "Question": "What is the central dogma of molecular biology?",
    "Answer": "A",
    "Explanation": "The central dogma describes the flow of genetic information: DNA is transcribed into RNA, which is translated into protein.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/2/2f/Central_dogma_of_molecular_biology.svg/1200px-Central_dogma_of_molecular_biology.svg.png",
    "OptionA": "DNA → RNA → Protein",
    "OptionB": "Protein → RNA → DNA",
    "OptionC": "RNA → DNA → Protein",
    "OptionD": "Protein → DNA → RNA",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Genetic Information Flow",
    "Item": 3,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which vitamin is essential for calcium absorption and bone health?",
    "Answer": "D",
    "Explanation": "Vitamin D helps the body absorb calcium, which is critical for maintaining strong bones.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/4/4f/Vitamin_D3.svg/1200px-Vitamin_D3.svg.png",
    "OptionA": "Vitamin A",
    "OptionB": "Vitamin C",
    "OptionC": "Vitamin B12",
    "OptionD": "Vitamin D",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Nutrition and Vitamins",
    "Item": 4,
    "Type": "multiple choice",
    "Path": "Nutrition"
  },
  {
    "Question": "Which enzyme is responsible for unwinding DNA during replication?",
    "Answer": "B",
    "Explanation": "Helicase unwinds the DNA double helix to allow replication machinery to copy each strand.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/7/7a/DNA_helicase.png/1200px-DNA_helicase.png",
    "OptionA": "Ligase",
    "OptionB": "Helicase",
    "OptionC": "Polymerase",
    "OptionD": "Primase",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "DNA Replication",
    "Item": 5,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which macronutrient provides the most energy per gram when metabolized?",
    "Answer": "C",
    "Explanation": "Fats provide about 9 kcal per gram, which is more than carbohydrates and proteins (about 4 kcal/g each).",
    "PictureURL": "",
    "OptionA": "Carbohydrates",
    "OptionB": "Proteins",
    "OptionC": "Fats",
    "OptionD": "Vitamins",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Nutrition and Energy",
    "Item": 6,
    "Type": "multiple choice",
    "Path": "Nutrition"
  },
  {
    "Question": "What is the function of messenger RNA (mRNA)?",
    "Answer": "A",
    "Explanation": "mRNA carries the genetic code from DNA in the nucleus to ribosomes in the cytoplasm for protein synthesis.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/3/3b/Messenger_RNA.svg/1200px-Messenger_RNA.svg.png",
    "OptionA": "Transmit genetic information from DNA to ribosomes",
    "OptionB": "Form the structure of ribosomes",
    "OptionC": "Carry amino acids to ribosomes",
    "OptionD": "Replicate DNA",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Molecular Biology - RNA Types",
    "Item": 7,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which metabolic pathway occurs in the mitochondria and produces the most ATP?",
    "Answer": "D",
    "Explanation": "The citric acid cycle (Krebs cycle) occurs in the mitochondria and generates electron carriers used in oxidative phosphorylation to produce ATP.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/9/9a/Citric_acid_cycle_with_aconitate_2.svg/1200px-Citric_acid_cycle_with_aconitate_2.svg.png",
    "OptionA": "Glycolysis",
    "OptionB": "Fermentation",
    "OptionC": "Pentose phosphate pathway",
    "OptionD": "Citric acid cycle",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Metabolism - Energy Production",
    "Item": 8,
    "Type": "multiple choice",
    "Path": "Biochemistry/Metabolism"
  },
  {
    "Question": "Which nutrient is a major source of nitrogen for the body?",
    "Answer": "B",
    "Explanation": "Proteins contain nitrogen in their amino groups, making them the primary nitrogen source in the diet.",
    "PictureURL": "",
    "OptionA": "Carbohydrates",
    "OptionB": "Proteins",
    "OptionC": "Fats",
    "OptionD": "Vitamins",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Nutrition - Macronutrients",
    "Item": 9,
    "Type": "multiple choice",
    "Path": "Nutrition"
  },
  {
    "Question": "What is the role of tRNA during protein synthesis?",
    "Answer": "C",
    "Explanation": "Transfer RNA (tRNA) brings specific amino acids to the ribosome to be added to the growing polypeptide chain.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/3/3f/Transfer_RNA.svg/1200px-Transfer_RNA.svg.png",
    "OptionA": "Carry genetic code from DNA",
    "OptionB": "Form ribosome structure",
    "OptionC": "Deliver amino acids to ribosomes",
    "OptionD": "Unwind DNA strands",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Molecular Biology - Protein Synthesis",
    "Item": 10,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which of the following is NOT a nucleotide base found in DNA?",
    "Answer": "D",
    "Explanation": "Uracil is found in RNA, not DNA. DNA contains adenine, thymine, cytosine, and guanine.",
    "PictureURL": "",
    "OptionA": "Adenine",
    "OptionB": "Thymine",
    "OptionC": "Cytosine",
    "OptionD": "Uracil",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Genetics - DNA Bases",
    "Item": 11,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which process converts RNA into protein?",
    "Answer": "B",
    "Explanation": "Translation is the process where ribosomes read mRNA sequences to synthesize proteins.",
    "PictureURL": "https://upload.wikimedia.org/wikipedia/commons/thumb/4/4e/Translation_process.svg/1200px-Translation_process.svg.png",
    "OptionA": "Replication",
    "OptionB": "Translation",
    "OptionC": "Transcription",
    "OptionD": "Splicing",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Molecular Biology - Protein Synthesis",
    "Item": 12,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which mineral is important for oxygen transport in the blood?",
    "Answer": "A",
    "Explanation": "Iron is a key component of hemoglobin, which carries oxygen in red blood cells.",
    "PictureURL": "",
    "OptionA": "Iron",
    "OptionB": "Calcium",
    "OptionC": "Potassium",
    "OptionD": "Magnesium",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Nutrition - Minerals",
    "Item": 13,
    "Type": "multiple choice",
    "Path": "Nutrition"
  },
  {
    "Question": "Which molecule acts as the template during transcription?",
    "Answer": "C",
    "Explanation": "DNA serves as the template for RNA synthesis during transcription.",
    "PictureURL": "",
    "OptionA": "mRNA",
    "OptionB": "tRNA",
    "OptionC": "DNA",
    "OptionD": "Protein",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Molecular Biology - Transcription",
    "Item": 14,
    "Type": "multiple choice",
    "Path": "Molecular Biology/Genetics"
  },
  {
    "Question": "Which metabolic process occurs in the absence of oxygen and produces lactic acid?",
    "Answer": "B",
    "Explanation": "Anaerobic glycolysis produces lactic acid when oxygen is scarce.",
    "PictureURL": "",
    "OptionA": "Aerobic respiration",
    "OptionB": "Fermentation",
    "OptionC": "Photosynthesis",
    "OptionD": "Beta-oxidation",
    "OptionE": "",
    "OptionF": "",
    "OptionG": "",
    "TestName": "Biochemistry and Molecular Biology Practice Test",
    "Content Type": "multiple choice",
    "Title": "Metabolism - Anaerobic Processes",
    "Item": 15,
    "Type": "multiple choice",
    "Path": "Biochemistry/Metabolism"
  }
]